To study ADENOSINE DEAMINASE activity in bovine tropical theileriosis, blood and serum sample were collected from 49 infected and 15 uninfected cattle. Haematological parameters were measured by standard methods in both groups. The results showed significant decrease in red blood cells (RBCs) count, hemoglobin and packed cell volume (PCV) which all indicating the occurance of anemia in infected animals. Furthermore, the infected cattle had higher lymphocyte, monocyte and eosinophil. Compared to uninfected, the infected animals showed higher ADENOSINE DEAMINASE activity. These findings along with lymphocytosis in peripheral blood circulation are showing an active immune response to infection and its related inflammatory reactions in different tissues. High levels of ADENOSINE DEAMINASE activity in infected cattle maybe regarded as an important index which needs more studies to elucidate its role in pathogenesis or immunopathogenesis in bovine tropical theileriosis.

Chronic obstructive pulmonary disease (COPD) has been defined by the Global Initiative for Chronic Obstructive Lung Disease (GOLD), as a disease state characterized by airflow limitation which is not fully reversible. COPD consists of emphysema which is the destruction and inflammation of the lung alveoli. ADENOSINE DEAMINASE (ADA, E.C.3.5.4.4) converts ADENOSINE to inosine. There are two isoenzymes of ADA in serum; ADA1 and ADA2. It has been established that in COPD patients the ADENOSINE levels increase, which can contribute to decrease of ADA activity. In this research we studied the ADA and its isoenzyme activity in COPD patients. This descriptive analytical case-control study was performed on thirty patients who were hospitalized in the pulmonary wards with an acute exacerbation of COPD. ADA activity was determined in 30 COPD patients, 30 nonsmokers and 30 smokers controls. All subjects were male. We used colorimetric (Giusti) method for measuring of ADA activity. The data were analyzed using SPSS 13 software and Kruskall-Wallis and two-way ANOVA tests.Total ADA activity in the COPD and smoker control groups was significantly lower than in non smoker group (18.99±7, 19.03±9.1 and 22.95±6.7 U/L, respectively). There was a significant difference for ADA2 between the three groups. Whereas the ADA1 activity in the three groups had no significant difference.Based on the obtained data, decrease of ADA activity may play an important role in the formation of pulmonary injury in COPD patients.

Background: Although tuberculosis is a known disease, still on occasion, the tuberculosis patients remain undiagnosed and hence not treated appropriately. In some cases, the patient is not able to give a proper sputum sample. In some other cases, expert laboratory technician to examine the patient’ s sputum smear is not available. Furthermore, and in most cases of extrapulmonary tuberculosis, invasive diagnostic procedures such as biopsy might be followed. The aim of this study was to investigate the diagnostic value of serum level of ADENOSINE DEAMINASE (ADA) in extra pulmonary tuberculosis. Methods: In this analytical study, pulmonary tuberculosis patients were compared with two other groups of patients including lung cancer patients and healthy controls, based on national tuberculosis protocol. In each group, 40 patients who almost matched for age and sex were included. Blood samples were taken from the participants to measure serum ADA levels. Data were analyzed using SPSS software version 15. 0. Results: Based on the data analyzed in this study, the mean ADA level in the patients with extra pulmonary tuberculosis was 23. 8 IU/L which was significantly higher than that in the other groups (P < 0. 001). Mean ADA levels in lung cancer patients and in healthy subjects were 15. 8 IU/L and 10. 7 IU/L, respectively. Conclusions: According to the results of this study, higher cut-off value for ADA would increase the specificity up to 100%. Moreover, serum ADA level can be a valuable additional index in diagnosis of extra pulmonary tuberculosis.